Live‐Animal Imaging of Renal Function by Multiphoton Microscopy

Brain Imaging with Multiphoton Microscopy

Multiphoton Microscopy for Ophthalmic Imaging

We review multiphoton microscopy (MPM) including two-photon autofluorescence (2PAF), second harmonic generation (SHG), third harmonic generation (THG), fluorescence lifetime (FLIM), and coherent anti-Stokes Raman Scattering (CARS) with relevance to clinical applications in ophthalmology. The different imaging modalities are discussed highlighting the particular strength that each has for functi...

Intravital multiphoton microscopy of dynamic renal processes.

Recent advances in microscopy and optics, computer sciences, and the available fluorophores used to label molecules of interest have empowered investigators to utilize intravital two-photon microscopy to study the dynamic events within the functioning kidney. This emerging technique enables investigators to follow functional and structural alterations with subcellular resolution within the same...

Functional Imaging of Photosensitizers using Multiphoton Microscopy

Multiphoton imaging of the sub-cellular distribution of photosensitizers can provide important clues to their mechanism of action in tumors. We have used this tool to study distribution and pharmacology of photosensitizers in murine hepatoma tumor cells dosed with various photosensitizers. Upon photoactivation, Rose Bengal yields nearly immediate photolytic release of lysosomal enzymes, resulti...

Multifocal Multiphoton Microscopy for Neurobiological Imaging

Observing neuronal structures and monitoring changes in synaptic connectivity with respect to time have a significant impact on understanding the basis of structural brain abnormalities and the development of therapeutics for their correction. Today, multiphoton excitation fluorescence microscopy is the method of choice for in vivo neuronal imaging with its inherent 3D resolution, minimal photo...